Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays. / Petersen, J.; Poulsen, L.; Petronis, S.; Birgens, H.; Dufva, M.
I: Nucleic Acids Research, Bind 36, Nr. 2, 2008, s. e10-.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays
AU - Petersen, J.
AU - Poulsen, L.
AU - Petronis, S.
AU - Birgens, H.
AU - Dufva, M.
N1 - Times Cited: 0ArticleEnglishDufva, MTech Univ Denmark, Dept Micro & Nanotechnol, Microarray Grp, Bld 345 E, DK-2800 Lyngby, DenmarkCited References Count: 38260PGOXFORD UNIV PRESSGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLANDOXFORD
PY - 2008
Y1 - 2008
N2 - DNA microarrays are generally operated at a single condition, which severely limits the freedom of designing probes for allele-specific hybridization assays. Here, we demonstrate a fluidic device for multi-stringency posthybridization washing of microarrays on microscope slides. This device is called a multi-thermal array washer (MTAW), and it has eight individually controlled heating zones, each of which corresponds to the location of a subarray on a slide. Allele-specific oligonucleotide probes for nine mutations in the beta-globin gene were spotted in eight identical subarrays at positions corresponding to the temperature zones of the MTAW. After hybridization with amplified patient material, the slides were mounted in the MTAW, and each subarray was exposed to different temperatures ranging from 22 to 40 degrees C. When processed in the MTAW, probes selected without considering melting temperature resulted in improved genotyping compared with probes selected according to theoretical melting temperature and run under one condition. In conclusion, the MTAW is a versatile tool that can facilitate screening of a large number of probes for genotyping assays and can also enhance the performance of diagnostic arrays Udgivelsesdato: 2008/2
AB - DNA microarrays are generally operated at a single condition, which severely limits the freedom of designing probes for allele-specific hybridization assays. Here, we demonstrate a fluidic device for multi-stringency posthybridization washing of microarrays on microscope slides. This device is called a multi-thermal array washer (MTAW), and it has eight individually controlled heating zones, each of which corresponds to the location of a subarray on a slide. Allele-specific oligonucleotide probes for nine mutations in the beta-globin gene were spotted in eight identical subarrays at positions corresponding to the temperature zones of the MTAW. After hybridization with amplified patient material, the slides were mounted in the MTAW, and each subarray was exposed to different temperatures ranging from 22 to 40 degrees C. When processed in the MTAW, probes selected without considering melting temperature resulted in improved genotyping compared with probes selected according to theoretical melting temperature and run under one condition. In conclusion, the MTAW is a versatile tool that can facilitate screening of a large number of probes for genotyping assays and can also enhance the performance of diagnostic arrays Udgivelsesdato: 2008/2
M3 - Journal article
VL - 36
SP - e10-
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 2
ER -
ID: 10246835