Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

  • J. Petersen
  • L. Poulsen
  • S. Petronis
  • H. Birgens
  • M. Dufva
DNA microarrays are generally operated at a single condition, which severely limits the freedom of designing probes for allele-specific hybridization assays. Here, we demonstrate a fluidic device for multi-stringency posthybridization washing of microarrays on microscope slides. This device is called a multi-thermal array washer (MTAW), and it has eight individually controlled heating zones, each of which corresponds to the location of a subarray on a slide. Allele-specific oligonucleotide probes for nine mutations in the beta-globin gene were spotted in eight identical subarrays at positions corresponding to the temperature zones of the MTAW. After hybridization with amplified patient material, the slides were mounted in the MTAW, and each subarray was exposed to different temperatures ranging from 22 to 40 degrees C. When processed in the MTAW, probes selected without considering melting temperature resulted in improved genotyping compared with probes selected according to theoretical melting temperature and run under one condition. In conclusion, the MTAW is a versatile tool that can facilitate screening of a large number of probes for genotyping assays and can also enhance the performance of diagnostic arrays
Udgivelsesdato: 2008/2
OriginalsprogEngelsk
TidsskriftNucleic Acids Research
Vol/bind36
Udgave nummer2
Sider (fra-til)e10-
ISSN0305-1048
StatusUdgivet - 2008

Bibliografisk note

Times Cited: 0ArticleEnglishDufva, MTech Univ Denmark, Dept Micro & Nanotechnol, Microarray Grp, Bld 345 E, DK-2800 Lyngby, DenmarkCited References Count: 38260PGOXFORD UNIV PRESSGREAT CLARENDON ST, OXFORD OX2 6DP, ENGLANDOXFORD

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