Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1

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Standard

Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1. / Kusk, P; Lindhardt, B O; Bugge, T H; Holmbäck, K; Hulgaard, E F.

I: Journal of Acquired Immune Deficiency Syndromes, Bind 6, Nr. 4, 04.1993, s. 334-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kusk, P, Lindhardt, BO, Bugge, TH, Holmbäck, K & Hulgaard, EF 1993, 'Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1', Journal of Acquired Immune Deficiency Syndromes, bind 6, nr. 4, s. 334-8.

APA

Kusk, P., Lindhardt, B. O., Bugge, T. H., Holmbäck, K., & Hulgaard, E. F. (1993). Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1. Journal of Acquired Immune Deficiency Syndromes, 6(4), 334-8.

Vancouver

Kusk P, Lindhardt BO, Bugge TH, Holmbäck K, Hulgaard EF. Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1. Journal of Acquired Immune Deficiency Syndromes. 1993 apr.;6(4):334-8.

Author

Kusk, P ; Lindhardt, B O ; Bugge, T H ; Holmbäck, K ; Hulgaard, E F. / Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1. I: Journal of Acquired Immune Deficiency Syndromes. 1993 ; Bind 6, Nr. 4. s. 334-8.

Bibtex

@article{57ad1e640bc6406eba7894dddef5f8dc,
title = "Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1",
abstract = "A random fragment expression library was used to identify and map a new human epitope on the vpu protein of the human immunodeficiency virus type 1 (HIV-1). The epitope was mapped to the central part of the protein within amino acids (aa) 37-50 comprising the sequence N-KIDRLIDRLI-ERAE-C. A alpha-galactosidase-vpu fusion protein representing aa 37-68 of vpu was used to screen 356 human serum samples from HIV-1-infected persons for antibodies to the novel epitope. A total of 125 (35.1%) of the samples reacted with this region of vpu. Antibodies against this region were significantly more prevalent among samples from individuals with CD4 cell counts < 400 cells/microliters than individuals with CD4 cell counts > or = 400 cells/microliters (37.6 vs. 17.6%; p < 0.0146, Fisher's exact test). Thus, the presence of antibodies against this epitope of vpu appears to be associated with a progressed state of disease.",
keywords = "Amino Acid Sequence, CD4-Positive T-Lymphocytes, Epitopes/analysis, Gene Library, HIV-1/genetics, Human Immunodeficiency Virus Proteins, Humans, Leukocyte Count, Male, Molecular Sequence Data, Viral Regulatory and Accessory Proteins/genetics",
author = "P Kusk and Lindhardt, {B O} and Bugge, {T H} and K Holmb{\"a}ck and Hulgaard, {E F}",
year = "1993",
month = apr,
language = "English",
volume = "6",
pages = "334--8",
journal = "J A I D S",
issn = "1525-4135",
publisher = "Lippincott Williams & Wilkins",
number = "4",

}

RIS

TY - JOUR

T1 - Mapping of a new immunodominant human linear B-cell epitope on the vpu protein of the human immunodeficiency virus type 1

AU - Kusk, P

AU - Lindhardt, B O

AU - Bugge, T H

AU - Holmbäck, K

AU - Hulgaard, E F

PY - 1993/4

Y1 - 1993/4

N2 - A random fragment expression library was used to identify and map a new human epitope on the vpu protein of the human immunodeficiency virus type 1 (HIV-1). The epitope was mapped to the central part of the protein within amino acids (aa) 37-50 comprising the sequence N-KIDRLIDRLI-ERAE-C. A alpha-galactosidase-vpu fusion protein representing aa 37-68 of vpu was used to screen 356 human serum samples from HIV-1-infected persons for antibodies to the novel epitope. A total of 125 (35.1%) of the samples reacted with this region of vpu. Antibodies against this region were significantly more prevalent among samples from individuals with CD4 cell counts < 400 cells/microliters than individuals with CD4 cell counts > or = 400 cells/microliters (37.6 vs. 17.6%; p < 0.0146, Fisher's exact test). Thus, the presence of antibodies against this epitope of vpu appears to be associated with a progressed state of disease.

AB - A random fragment expression library was used to identify and map a new human epitope on the vpu protein of the human immunodeficiency virus type 1 (HIV-1). The epitope was mapped to the central part of the protein within amino acids (aa) 37-50 comprising the sequence N-KIDRLIDRLI-ERAE-C. A alpha-galactosidase-vpu fusion protein representing aa 37-68 of vpu was used to screen 356 human serum samples from HIV-1-infected persons for antibodies to the novel epitope. A total of 125 (35.1%) of the samples reacted with this region of vpu. Antibodies against this region were significantly more prevalent among samples from individuals with CD4 cell counts < 400 cells/microliters than individuals with CD4 cell counts > or = 400 cells/microliters (37.6 vs. 17.6%; p < 0.0146, Fisher's exact test). Thus, the presence of antibodies against this epitope of vpu appears to be associated with a progressed state of disease.

KW - Amino Acid Sequence

KW - CD4-Positive T-Lymphocytes

KW - Epitopes/analysis

KW - Gene Library

KW - HIV-1/genetics

KW - Human Immunodeficiency Virus Proteins

KW - Humans

KW - Leukocyte Count

KW - Male

KW - Molecular Sequence Data

KW - Viral Regulatory and Accessory Proteins/genetics

M3 - Journal article

C2 - 7681110

VL - 6

SP - 334

EP - 338

JO - J A I D S

JF - J A I D S

SN - 1525-4135

IS - 4

ER -

ID: 201190515