Elevated baseline salivary protease activity may predict the steadiness of gingival inflammation during periodontal healing: a 12-week follow-up study on adults
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Elevated baseline salivary protease activity may predict the steadiness of gingival inflammation during periodontal healing : a 12-week follow-up study on adults. / Gürsoy, Ulvi Kahraman; Fteita, Dareen; Bikker, Floris J.; Grande, Maria Anastasia; Nazmi, Kamran; Gürsoy, Mervi; Könönen, Eija; Belstrøm, Daniel.
I: Pathogens, Bind 9, Nr. 9, 751, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Elevated baseline salivary protease activity may predict the steadiness of gingival inflammation during periodontal healing
T2 - a 12-week follow-up study on adults
AU - Gürsoy, Ulvi Kahraman
AU - Fteita, Dareen
AU - Bikker, Floris J.
AU - Grande, Maria Anastasia
AU - Nazmi, Kamran
AU - Gürsoy, Mervi
AU - Könönen, Eija
AU - Belstrøm, Daniel
PY - 2020
Y1 - 2020
N2 - Aim was to profile salivary total protease, Porphyromonas gingivalis gingipain, and neutrophil elastase activities in relation to the resolution of periodontal inflammation, salivary macrophage-derived chemokine (MDC), and macrophage inflammatory protein-1α concentrations. Nonsurgical periodontal treatment was performed in 24 periodontitis patients in a prospective interventional study design. Periodontal clinical parameters were recorded, and stimulated saliva samples were collected at baseline and 2, 6, and 12 weeks after treatment. Salivary total protease and gingipain activities were determined using fluorogenic substrates, elastase activity by chromogenic substrates, and cytokine concentrations by Luminex immunoassay. For statistical analyses, generalized linear mixed models for repeated measures were used. Salivary total protease activity elevated, while gingival inflammation and plaque accumulation decreased 2 and 6 weeks after periodontal therapy. Salivary MDC concentration was elevated 12 weeks after periodontal treatment. Patients with elevated protease activities at baseline in comparison to patients with low baseline total protease activities, had higher levels of gingival inflammation before and after periodontal treatment. In conclusion, elevations in salivary total protease activity seem to be part of periodontal healing at its early phases. Higher levels of salivary total protease activities before periodontal treatment may predict the severity and steadiness of unresolved gingival inflammation.
AB - Aim was to profile salivary total protease, Porphyromonas gingivalis gingipain, and neutrophil elastase activities in relation to the resolution of periodontal inflammation, salivary macrophage-derived chemokine (MDC), and macrophage inflammatory protein-1α concentrations. Nonsurgical periodontal treatment was performed in 24 periodontitis patients in a prospective interventional study design. Periodontal clinical parameters were recorded, and stimulated saliva samples were collected at baseline and 2, 6, and 12 weeks after treatment. Salivary total protease and gingipain activities were determined using fluorogenic substrates, elastase activity by chromogenic substrates, and cytokine concentrations by Luminex immunoassay. For statistical analyses, generalized linear mixed models for repeated measures were used. Salivary total protease activity elevated, while gingival inflammation and plaque accumulation decreased 2 and 6 weeks after periodontal therapy. Salivary MDC concentration was elevated 12 weeks after periodontal treatment. Patients with elevated protease activities at baseline in comparison to patients with low baseline total protease activities, had higher levels of gingival inflammation before and after periodontal treatment. In conclusion, elevations in salivary total protease activity seem to be part of periodontal healing at its early phases. Higher levels of salivary total protease activities before periodontal treatment may predict the severity and steadiness of unresolved gingival inflammation.
KW - Chemokine CCL22
KW - Macrophage inflammatory proteins
KW - Periodontitis
KW - Porphyromonas gingivalis
KW - Saliva
U2 - 10.3390/pathogens9090751
DO - 10.3390/pathogens9090751
M3 - Journal article
C2 - 32942694
AN - SCOPUS:85090897830
VL - 9
JO - Pathogens
JF - Pathogens
SN - 2076-0817
IS - 9
M1 - 751
ER -
ID: 249064575