Effect of prior dietary exposure to cows' milk protein on antigen-specific and nonspecific cellular proliferation in mice
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Effect of prior dietary exposure to cows' milk protein on antigen-specific and nonspecific cellular proliferation in mice. / Brix, Susanne; Magyar, Orit H.; Barkholt, Vibeke; Frøkiær, Hanne.
I: Journal of Dairy Research, Bind 72, Nr. 2, 05.2005, s. 217-225.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Effect of prior dietary exposure to cows' milk protein on antigen-specific and nonspecific cellular proliferation in mice
AU - Brix, Susanne
AU - Magyar, Orit H.
AU - Barkholt, Vibeke
AU - Frøkiær, Hanne
PY - 2005/5
Y1 - 2005/5
N2 - The impact of dietary components on the immune system is gaining increased attention in the effort to develop safe food products, some even with health-promoting potential, as well as to improve the basic understanding of the immunomodulatory potential of common food components. In such studies, which are mainly based on experiments in vitro, it is important to be able to differentiate nonspecific activation of immune cells induced by dietary components from ex vivo restimulation of antigen-specific cells that might be present in cell cultures owing to prior dietary exposure to the antigens in cell donors. Focusing on the immunostimulatory potential of cows' milk proteins and peptides, we studied the impact of prior dietary exposure to cows' milk on proliferation of murine immune cells upon ex vivo stimulation with bovine milk proteins. Nonspecific proliferation induced by β-casein peptides was further assessed on cells from mice bred on a cows'-milk-free diet. Regarding the dietary effect, we found that prior oral intake of cows' milk proteins affected cell proliferation induced by culturing with cows' milk proteins in vitro, as spleen cells from mice fed a milk-containing diet showed a significantly greater proliferative response than did cells from mice bred on a cows'-milk-free diet. Studies of immune enhancing potentials of β-casein peptides showed that some peptides stimulate proliferation of immune cells nonspecifically. In conclusion, these findings stress the importance of employing immune cells from mice unexposed to cows' milk for studies of the immunomodulating capacity of cows' milk proteins and peptides, in order to rule out the interference caused by antigen-specific immune responses. By using such cells, we here show that some β-casein peptides possess the potential to induce proliferation in immune cells in a nonspecific manner.
AB - The impact of dietary components on the immune system is gaining increased attention in the effort to develop safe food products, some even with health-promoting potential, as well as to improve the basic understanding of the immunomodulatory potential of common food components. In such studies, which are mainly based on experiments in vitro, it is important to be able to differentiate nonspecific activation of immune cells induced by dietary components from ex vivo restimulation of antigen-specific cells that might be present in cell cultures owing to prior dietary exposure to the antigens in cell donors. Focusing on the immunostimulatory potential of cows' milk proteins and peptides, we studied the impact of prior dietary exposure to cows' milk on proliferation of murine immune cells upon ex vivo stimulation with bovine milk proteins. Nonspecific proliferation induced by β-casein peptides was further assessed on cells from mice bred on a cows'-milk-free diet. Regarding the dietary effect, we found that prior oral intake of cows' milk proteins affected cell proliferation induced by culturing with cows' milk proteins in vitro, as spleen cells from mice fed a milk-containing diet showed a significantly greater proliferative response than did cells from mice bred on a cows'-milk-free diet. Studies of immune enhancing potentials of β-casein peptides showed that some peptides stimulate proliferation of immune cells nonspecifically. In conclusion, these findings stress the importance of employing immune cells from mice unexposed to cows' milk for studies of the immunomodulating capacity of cows' milk proteins and peptides, in order to rule out the interference caused by antigen-specific immune responses. By using such cells, we here show that some β-casein peptides possess the potential to induce proliferation in immune cells in a nonspecific manner.
KW - β-Casein
KW - Immunostimulation
KW - Peptides
UR - http://www.scopus.com/inward/record.url?scp=18844405223&partnerID=8YFLogxK
U2 - 10.1017/S0022029905000877
DO - 10.1017/S0022029905000877
M3 - Journal article
C2 - 15909688
AN - SCOPUS:18844405223
VL - 72
SP - 217
EP - 225
JO - Journal of Dairy Research
JF - Journal of Dairy Research
SN - 0022-0299
IS - 2
ER -
ID: 316998663