Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig
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Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig. / Schmidt, P T; Hansen, L; Hilsted, L; Holst, Jens Juul.
I: Scandinavian Journal of Gastroenterology, Bind 39, Nr. 3, 03.2004, s. 217-21.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Cholecystokinin inhibits gastrin secretion independently of paracrine somatostatin secretion in the pig
AU - Schmidt, P T
AU - Hansen, L
AU - Hilsted, L
AU - Holst, Jens Juul
PY - 2004/3
Y1 - 2004/3
N2 - BACKGROUND: Cholecystokinin inhibits the secretion of gastrin from antral G cells, an effect that is speculated to be mediated by D cells secreting somatostatin. The aim of the study was to test directly whether cholecystokinin inhibition of antral gastrin secretion is mediated by somatostatin.METHODS: The effects of CCK on gastrin and somatostatin secretion were studied in isolated vascularly perfused preparations of pig antrum before and after immunoneutralization brought about by infusion of large amounts of a high affinity monoclonal antibody against somatostatin.RESULTS: CCK infusion at 10(-9) M and 10(-8) M decreased gastrin output to 70.5% +/- 7.6% (n = 8) and 76.3% +/- 3.6% (n = 7) of basal output, respectively. CCK at 10(-10) M had no effect (n = 6). Somatostatin secretion was dose-dependently increased by CCK infusion and increased to 268 +/- 38.2% (n = 7) of basal secretion during infusion of CCK at 10(-8) M. Immunoneutralization of somatostatin caused a doubling of the basal secretion of gastrin, but did not affect the CCK-induced decrease in gastrin secretion.CONCLUSION: CCK inhibits gastrin secretion independently of paracrine somatostatin secretion.
AB - BACKGROUND: Cholecystokinin inhibits the secretion of gastrin from antral G cells, an effect that is speculated to be mediated by D cells secreting somatostatin. The aim of the study was to test directly whether cholecystokinin inhibition of antral gastrin secretion is mediated by somatostatin.METHODS: The effects of CCK on gastrin and somatostatin secretion were studied in isolated vascularly perfused preparations of pig antrum before and after immunoneutralization brought about by infusion of large amounts of a high affinity monoclonal antibody against somatostatin.RESULTS: CCK infusion at 10(-9) M and 10(-8) M decreased gastrin output to 70.5% +/- 7.6% (n = 8) and 76.3% +/- 3.6% (n = 7) of basal output, respectively. CCK at 10(-10) M had no effect (n = 6). Somatostatin secretion was dose-dependently increased by CCK infusion and increased to 268 +/- 38.2% (n = 7) of basal secretion during infusion of CCK at 10(-8) M. Immunoneutralization of somatostatin caused a doubling of the basal secretion of gastrin, but did not affect the CCK-induced decrease in gastrin secretion.CONCLUSION: CCK inhibits gastrin secretion independently of paracrine somatostatin secretion.
KW - Animals
KW - Cholecystokinin
KW - Gastrin-Secreting Cells
KW - Gastrins
KW - Paracrine Communication
KW - Pyloric Antrum
KW - Radioimmunoassay
KW - Somatostatin
KW - Somatostatin-Secreting Cells
KW - Swine
M3 - Journal article
C2 - 15074389
VL - 39
SP - 217
EP - 221
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 0036-5521
IS - 3
ER -
ID: 132054559